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Home>Product>Primary Antibody

ATP2A2 Recombinant Rabbit mAb

#4296

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Recombinant Antibody
Western blotting analysis using ATP2A2 antibody (Cat#4296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A2 antibody (Cat#4296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with ATP2A2 antibody (Cat#4296, 1:1,000). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP2A2 expression in HeLa cells using ATP2A2 antibody (Cat#4296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2A2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP2A2
  • Catalog：
    4296
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ATP2A2 Recombinant Rabbit mAb
Aliases ATP2A2; ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 2; SERCA2; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2; Calcium Pump 2; ATP2B; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SR Ca(2+)-ATPase; DAR; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Slow Twitch Skeletal Muscle Isoform; ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2; ATPase Ca++ Transporting Cardiac Muscle Slow Twitch 2; ATPase, Ca++ Dependent, Slow-Twitch, Cardiac Muscle-2; Cardiac Ca2+ ATPase; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3; DD
Background

Gene Name: ATP2A2

NCBI Gene Entry: 488

UniProt Entry: P16615

Application Information

Molecular Weight: Predicted, 115 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10510

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SERCA2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP2A2 antibody (Cat#4296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A2 antibody (Cat#4296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with ATP2A2 antibody (Cat#4296, 1:1,000). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ATP2A2 expression in HeLa cells using ATP2A2 antibody (Cat#4296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2A2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP2A2
  • Catalog：
    4296
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP2A2 antibody (Cat#4296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A2 antibody (Cat#4296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with ATP2A2 antibody (Cat#4296, 1:1,000). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP2A2 expression in HeLa cells using ATP2A2 antibody (Cat#4296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2A2.
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