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Home>Product>Primary Antibody

NAD(P)H Quinone Dehydrogenase 1 Recombinant Rabbit mAb

#4301

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Recombinant Antibody
Western blotting analysis using NQO1 antibody (Cat#4301). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NQO1 antibody (Cat#4301, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NQO1 antibody (Cat#4301, 1:1,000). Nuclei were stained blue with DAPI; NQO1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NQO1 expression in HT-1080 cells using NQO1 antibody (Cat#4301, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NQO1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NQO1
  • Catalog：
    4301
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NAD(P)H Quinone Dehydrogenase 1 Recombinant Rabbit mAb
Aliases NAD(P)H Quinone Dehydrogenase 1; QR1; DTD; DT-Diaphorase; NMOR1; DHQU; DIA4; Diaphorase (NADH/NADPH) (Cytochrome B-5 Reductase); NAD(P)H Dehydrogenase [Quinone] 1; NAD(P)H Dehydrogenase, Quinone 1; NAD(P)H:Quinone Oxidoreductase 1; NAD(P)H-Quinone Oxidoreductase; Phylloquinone Reductase; Menadione Reductase; Quinone Reductase 1; Azoreductase; EC 1.6.5.2; NAD(P)H:Quinone Acceptor Oxidoreductase Type 1; NAD(P)H:Menadione Oxidoreductase 1; NAD(P)H:Quinone Oxireductase; Dioxin-Inducible 1; Quinone Reductase; Diaphorase-4; NMORI
Background

Gene Name: NQO1

NCBI Gene Entry: 1728

UniProt Entry: P15559

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 29 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10535

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NQO1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NQO1 antibody (Cat#4301). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NQO1 antibody (Cat#4301, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with NQO1 antibody (Cat#4301, 1:1,000). Nuclei were stained blue with DAPI; NQO1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NQO1 expression in HT-1080 cells using NQO1 antibody (Cat#4301, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NQO1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NQO1
  • Catalog：
    4301
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NQO1 antibody (Cat#4301). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NQO1 antibody (Cat#4301, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NQO1 antibody (Cat#4301, 1:1,000). Nuclei were stained blue with DAPI; NQO1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NQO1 expression in HT-1080 cells using NQO1 antibody (Cat#4301, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NQO1.
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