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Carbonic Anhydrase II Recombinant Rabbit mAb

#4353

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Recombinant Antibody
Western blotting analysis using carbonic anhydrase II antibody (Cat#4353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase II antibody (Cat#4353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Carbonic anhydrase II expression in HeLa cells using Carbonic anhydrase II antibody (Cat#4353, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Carbonic anhydrase II.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CA2
  • Catalog:
    4353
  • Application:
    WB, FCM
  • Species Reactivity:
    H, R
Information
Product Name Carbonic Anhydrase II Recombinant Rabbit mAb
Aliases CA2; Carbonic Anhydrase 2; CA-II; Carbonic Anhydrase II; CAII; Carbonate Dehydratase II; Cyanamide Hydratase CA2; Carbonic Anhydrase C; EC 4.2.1.1; Car2; CAC; Epididymis Secretory Protein Li 282; Epididymis Luminal Protein 76; Carbonic Anhydrase B; Carbonic Dehydratase; EC 4.2.1.69; HEL-S-282; HEL-76; CAR2
Background

Gene Name: CA2

NCBI Gene Entry: 760

UniProt Entry: P00918

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 29 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10795

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Carbonic anhydrase 2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using carbonic anhydrase II antibody (Cat#4353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase II antibody (Cat#4353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Carbonic anhydrase II expression in HeLa cells using Carbonic anhydrase II antibody (Cat#4353, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Carbonic anhydrase II.
Western blotting analysis using carbonic anhydrase II antibody (Cat#4353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase II antibody (Cat#4353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Carbonic anhydrase II expression in HeLa cells using Carbonic anhydrase II antibody (Cat#4353, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Carbonic anhydrase II.
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