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Home>Product>Primary Antibody

Zinc Finger E-Box Binding Homeobox 1 Recombinant Rabbit mAb

#4396

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Recombinant Antibody
Western blotting analysis using ZEB1 antibody (Cat#4396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZEB1 antibody (Cat#4396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ZEB1 antibody (Cat#4396, 1: 1,000). Nuclei were stained blue with DAPI; ZEB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZEB1 expression in C2C12 cells using ZEB1 antibody (Cat#4396, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZEB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ZEB1
  • Catalog：
    4396
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Zinc Finger E-Box Binding Homeobox 1 Recombinant Rabbit mAb
Aliases ZEB1; Zinc Finger E-Box Binding Homeobox 1; AREB6; Zfhx1a; Zfhep; FECD6; TCF8; BZP; Transcription Factor 8 (Represses Interleukin 2 Expression); Posterior Polymorphous Corneal Dystrophy 3; Zinc Finger E-Box-Binding Homeobox 1; Negative Regulator Of IL2; NIL-2-A; PPCD3; ZEB; Zinc Finger Homeodomain Enhancer-Binding Protein; Delta-Crystallin Enhancer Binding Factor 1; NIL-2-A Zinc Finger Protein; Transcription Factor 8; DELTAEF1; NIL2A; TCF-8
Background

Gene Name: ZEB1

NCBI Gene Entry: 6935

UniProt Entry: P37275

Application Information

Molecular Weight: Predicted, 124 kDa; observed, 200 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11010

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human AREB6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ZEB1 antibody (Cat#4396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZEB1 antibody (Cat#4396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with ZEB1 antibody (Cat#4396, 1: 1,000). Nuclei were stained blue with DAPI; ZEB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ZEB1 expression in C2C12 cells using ZEB1 antibody (Cat#4396, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZEB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ZEB1
  • Catalog：
    4396
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using ZEB1 antibody (Cat#4396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZEB1 antibody (Cat#4396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ZEB1 antibody (Cat#4396, 1: 1,000). Nuclei were stained blue with DAPI; ZEB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZEB1 expression in C2C12 cells using ZEB1 antibody (Cat#4396, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZEB1.
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