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Home>Product>Primary Antibody

BCAR1 Recombinant Rabbit mAb

#4524

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Recombinant Antibody
Western blotting analysis using BCAR1 antibody (Cat#4524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#4524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with BCAR1 antibody (Cat#4524, 1:1,000). Nuclei were stained blue with DAPI; BCAR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCAR1 expression in H9C2 cells using BCAR1 antibody (Cat#4524, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCAR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCAR1
  • Catalog：
    4524
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name BCAR1 Recombinant Rabbit mAb
Aliases BCAR1; BCAR1 Scaffold Protein, Cas Family Member; CASS1; CAS; Cas Scaffolding Protein Family Member 1; CRKAS; Breast Cancer Anti-Estrogen Resistance Protein 1; BCAR1, Cas Family Scaffolding Protein; BCAR1, Cas Family Scaffold Protein; P130Cas; Breast Cancer Anti-Estrogen Resistance 1; Crk-Associated Substrate P130Cas; Crk-Associated Substrate; CRK-Associated Substrate; P130CAS; P130cas; Crkas; CAS1
Background

Gene Name: BCAR1

NCBI Gene Entry: 9564

UniProt Entry: P56945

Application Information

Molecular Weight: Predicted, 93 kDa; observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11650

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human BCAR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using BCAR1 antibody (Cat#4524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#4524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9c2 cells with BCAR1 antibody (Cat#4524, 1:1,000). Nuclei were stained blue with DAPI; BCAR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of BCAR1 expression in H9C2 cells using BCAR1 antibody (Cat#4524, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCAR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCAR1
  • Catalog：
    4524
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using BCAR1 antibody (Cat#4524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#4524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with BCAR1 antibody (Cat#4524, 1:1,000). Nuclei were stained blue with DAPI; BCAR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCAR1 expression in H9C2 cells using BCAR1 antibody (Cat#4524, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCAR1.
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