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Home>Product>Primary Antibody

Calpain Small Subunit 1 Recombinant Rabbit mAb

#4637

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Recombinant Antibody
Western blotting analysis using calpain small subunit 1 antibody (Cat#4637). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain small subunit 1 antibody (Cat#4637, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody (Cat#4637, 1:1,000). Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody (Cat#4637, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Calpain small subunit 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CAPNS1
  • Catalog：
    4637
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Calpain Small Subunit 1 Recombinant Rabbit mAb
Aliases CAPNS1; Calpain Small Subunit 1; CDPS; CANPS; CAPN4; CANP; Calcium-Activated Neutral Proteinase Small Subunit; Calcium-Dependent Protease Small Subunit 1; Calpain Regulatory Subunit; CANP Small Subunit; CSS1; 30K; Epididymis Secretory Sperm Binding Protein; Calcium-Dependent Protease, Small Subunit; Calcium-Dependent Protease Small Subunit; Calpain 4, Small Subunit (30K); Calpain, Small Polypeptide; CALPAIN4; CAPNS
Background

Gene Name: CAPNS1

NCBI Gene Entry: 826

UniProt Entry: P04632

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12195

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Calpain small subunit 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using calpain small subunit 1 antibody (Cat#4637). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain small subunit 1 antibody (Cat#4637, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody (Cat#4637, 1:1,000). Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody (Cat#4637, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Calpain small subunit 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CAPNS1
  • Catalog：
    4637
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using calpain small subunit 1 antibody (Cat#4637). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain small subunit 1 antibody (Cat#4637, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody (Cat#4637, 1:1,000). Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody (Cat#4637, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Calpain small subunit 1.
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