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Home>Product>Primary Antibody

NDUFAF5 Recombinant Rabbit mAb

#4906

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Recombinant Antibody
Western blotting analysis using NDUFAF5 antibody (Cat#4906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFAF5 antibody (Cat#4906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with NDUFAF5 antibody (Cat#4906, 1:1,000). Nuclei were stained blue with DAPI; NDUFAF5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFAF5 expression in C2C12 cells using NDUFAF5 antibody (Cat#4906, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFAF5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFAF5
  • Catalog：
    4906
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name NDUFAF5 Recombinant Rabbit mAb
Aliases NDUFAF5; NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 5; DJ842G6.1; C20orf7; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 5; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 5; Arginine-Hydroxylase NDUFAF5, Mitochondrial; Putative Methyltransferase NDUFAF5; Probable Methyltransferase C20orf7, Mitochondrial; Chromosome 20 Open Reading Frame 7; BA526K24.2; EC 1.-.-.- ; EC 2.1.1.- ; MC1DN16
Background

Gene Name: NDUFAF5

NCBI Gene Entry: 79133

UniProt Entry: Q5TEU4

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 39 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13690

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NDUFAF5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFAF5 antibody (Cat#4906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFAF5 antibody (Cat#4906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with NDUFAF5 antibody (Cat#4906, 1:1,000). Nuclei were stained blue with DAPI; NDUFAF5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NDUFAF5 expression in C2C12 cells using NDUFAF5 antibody (Cat#4906, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFAF5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFAF5
  • Catalog：
    4906
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using NDUFAF5 antibody (Cat#4906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFAF5 antibody (Cat#4906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with NDUFAF5 antibody (Cat#4906, 1:1,000). Nuclei were stained blue with DAPI; NDUFAF5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFAF5 expression in C2C12 cells using NDUFAF5 antibody (Cat#4906, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFAF5.
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