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Home>Product>Primary Antibody

Phospho-Filamin A/B (S2152/S2107) Recombinant Rabbit mAb

#4920

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Recombinant Antibody
Western blotting analysis using phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Filamin A/B (Ser2152/Ser2107) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Filamin A/B (Ser2152/Ser2107) expression in C2C12 cells using Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A/B (Ser2152/Ser2107).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FLNB
  • Catalog：
    4920
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Filamin A/B (S2152/S2107) Recombinant Rabbit mAb
Aliases FLNB; Filamin B; ABP-278; TABP; TAP; FLN1L; FH1; Larsen Syndrome 1 (Autosomal Dominant); Actin-Binding-Like Protein; Actin Binding Protein 278; Thyroid Autoantigen; Filamin Homolog 1; Filamin B, Beta; ABP-280 Homolog; Beta-Filamin; Filamin-B; Filamin-3; FLN-B; LRS1; Filamin B, Beta (Actin Binding Protein 278); Truncated Actin-Binding Protein; Truncated ABP; Beta Filamin; ABP-280; FLN3; AOI; SCT; Fh1
Background

Gene Name: FLNB

NCBI Gene Entry: 2317

UniProt Entry: O75369

Application Information

Molecular Weight: Predicted, 278 kDa; observed, 281 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13760

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Filamin A/B (Ser2152/Ser2107)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Filamin A/B (Ser2152/Ser2107) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Filamin A/B (Ser2152/Ser2107) expression in C2C12 cells using Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A/B (Ser2152/Ser2107).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FLNB
  • Catalog：
    4920
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Filamin A/B (Ser2152/Ser2107) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Filamin A/B (Ser2152/Ser2107) expression in C2C12 cells using Phospho-Filamin A/B (Ser2152/Ser2107) antibody (Cat#4920, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A/B (Ser2152/Ser2107).
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