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Home>Product>Primary Antibody

SMARCA2 Recombinant Rabbit mAb

#4930

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Recombinant Antibody
Western blotting analysis using SMARCA2 antibody (Cat#4930). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA2 antibody (Cat#4930, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with SMARCA2 antibody (Cat#4930, 1:1,000). Nuclei were stained blue with DAPI; SMARCA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCA2 expression in HepG2 cells using SMARCA2 antibody (Cat#4930, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCA2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCA2
  • Catalog：
    4930
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name SMARCA2 Recombinant Rabbit mAb
Aliases SMARCA2; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2; HBRM; BRM; BAF190; HSNF2a; SNF2LA; SNF2L2; Sth1p; SNF2; SWI2; Probable Global Transcription Activator SNF2L2; ATP-Dependent Helicase SMARCA2; BRG1-Associated Factor 190B; Protein Brahma Homolog; Brahma Homolog; SNF2-Alpha; BAF190B; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin A2; Global Transcription Activator Homologous Sequence; Putative Global Transcription Activator SNF2L2; Sucrose Nonfermenting 2-Like Protein 2; SNF2/SWI2-Like Protein 2; EC 3.6.4.-; EC 3.6.1 47NCBRS; SNF2A; BIS
Background

Gene Name: SMARCA2

NCBI Gene Entry: 6595

UniProt Entry: P51531

Application Information

Molecular Weight: Predicted, 181 kDa; observed, 210 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13810

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SMARCA2/BRM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCA2 antibody (Cat#4930). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA2 antibody (Cat#4930, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with SMARCA2 antibody (Cat#4930, 1:1,000). Nuclei were stained blue with DAPI; SMARCA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SMARCA2 expression in HepG2 cells using SMARCA2 antibody (Cat#4930, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCA2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCA2
  • Catalog：
    4930
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using SMARCA2 antibody (Cat#4930). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA2 antibody (Cat#4930, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with SMARCA2 antibody (Cat#4930, 1:1,000). Nuclei were stained blue with DAPI; SMARCA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCA2 expression in HepG2 cells using SMARCA2 antibody (Cat#4930, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCA2.
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