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Home>Product>Primary Antibody

PTPRC Mouse mAb

#5048

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Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD45.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTPRC
  • Catalog：
    5048
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name PTPRC Mouse mAb
Aliases PTPRC; Protein Tyrosine Phosphatase Receptor Type C; T200; GP180; CD45; LCA; Receptor-Type Tyrosine-Protein Phosphatase C; Glycoprotein 180; CD45 Antigen; CD45R; B220; L-CA; LY5; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; T200 Leukocyte Common Antigen; Leukocyte-Common Antigen; Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; IMD105
Background

Gene Name: PTPRC

NCBI Gene Entry: 5788

UniProt Entry: P08575

Application Information

Molecular Weight: Predicted, 147 kDa; observed, 180-240 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14030

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human PTPRC
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100
Note This product is for research use only.
Data
  • Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD45.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTPRC
  • Catalog：
    5048
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD45.
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