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Home>Product>Primary Antibody

WWP1 Mouse Oligoclonal Antibody

#5149

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Western blotting analysis using WWP1 antibody (Cat#5149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WWP1 antibody (Cat#5149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with WWP1 antibody (Cat#5149, 1:1,000). Nuclei were stained blue with DAPI; WWP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of WWP1 expression in HepG2 cells using WWP1 antibody (Cat#5149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, WWP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WWP1
  • Catalog：
    5149
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name WWP1 Mouse Oligoclonal Antibody
Aliases WWP1; WW Domain Containing E3 Ubiquitin Protein Ligase 1; AIP5; NEDD4-Like E3 Ubiquitin-Protein Ligase WWP1; HECT-Type E3 Ubiquitin Transferase WWP1; TGIF-Interacting Ubiquitin Ligase 1; WW Domain-Containing Protein 1; DKFZP434D2111; Tiul1; Nedd-4-Like Ubiquitin-Protein Ligase; Atrophin-1 Interacting Protein 5; Atrophin-1-Interacting Protein 5; EC 2.3.2.26; EC 6.3.2; HSDRP1
Background

Gene Name: WWP1

NCBI Gene Entry: 11059

UniProt Entry: Q9H0M0

Application Information

Molecular Weight: Predicted, 105 kDa; observed, 125 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human WWP1
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using WWP1 antibody (Cat#5149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WWP1 antibody (Cat#5149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with WWP1 antibody (Cat#5149, 1:1,000). Nuclei were stained blue with DAPI; WWP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of WWP1 expression in HepG2 cells using WWP1 antibody (Cat#5149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, WWP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WWP1
  • Catalog：
    5149
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using WWP1 antibody (Cat#5149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WWP1 antibody (Cat#5149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with WWP1 antibody (Cat#5149, 1:1,000). Nuclei were stained blue with DAPI; WWP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of WWP1 expression in HepG2 cells using WWP1 antibody (Cat#5149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, WWP1.
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