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Home>Product>Primary Antibody

Prostaglandin D2 Synthase Recombinant Rabbit mAb

#5215

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Recombinant Antibody
Western blotting analysis using prostaglandin D2 synthase antibody (Cat#5215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with prostaglandin D2 synthase antibody (Cat#5215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Prostaglandin D2 synthase antibody (Cat#5215, 1:1,000). Nuclei were stained blue with DAPI; Prostaglandin D2 synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Prostaglandin D2 synthase expression in HepG2 cells using Prostaglandin D2 synthase antibody (Cat#5215, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Prostaglandin D2 synthase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTGDS
  • Catalog：
    5215
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Prostaglandin D2 Synthase Recombinant Rabbit mAb
Aliases PTGDS; Prostaglandin D2 Synthase; L-PGDS; PGDS; Lipocalin-Type Prostaglandin D Synthase; Prostaglandin D2 Synthase 21kDa (Brain); Glutathione-Independent PGD Synthase; Prostaglandin-H2 D-Isomerase; Beta-Trace Protein; PGD2 Synthase; Cerebrin-28; EC 5.3.99.2; PGDS2; PDS; Testis Tissue Sperm-Binding Protein Li 63n; Prostaglandin D2 Synthase (21kD, Brain); Lipocalin-Type Prostaglandin-D Synthase; Glutathione-Independent PGD Synthetase; Prostaglandin-D2 Synthase; Prostaglandin D Synthase; LPGDS; PGD2
Background

Gene Name: PTGDS

NCBI Gene Entry: 5730

UniProt Entry: P41222

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB200

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Prostaglandin D Synthase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using prostaglandin D2 synthase antibody (Cat#5215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with prostaglandin D2 synthase antibody (Cat#5215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Prostaglandin D2 synthase antibody (Cat#5215, 1:1,000). Nuclei were stained blue with DAPI; Prostaglandin D2 synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Prostaglandin D2 synthase expression in HepG2 cells using Prostaglandin D2 synthase antibody (Cat#5215, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Prostaglandin D2 synthase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTGDS
  • Catalog：
    5215
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using prostaglandin D2 synthase antibody (Cat#5215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with prostaglandin D2 synthase antibody (Cat#5215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Prostaglandin D2 synthase antibody (Cat#5215, 1:1,000). Nuclei were stained blue with DAPI; Prostaglandin D2 synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Prostaglandin D2 synthase expression in HepG2 cells using Prostaglandin D2 synthase antibody (Cat#5215, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Prostaglandin D2 synthase.
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