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Home>Product>Primary Antibody

SCD Recombinant Rabbit mAb

#5218

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Recombinant Antibody
Western blotting analysis using stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000). Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Stearoyl-CoA desaturase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    SCD
  • Catalog：
    5218
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name SCD Recombinant Rabbit mAb
Aliases SCD; Stearoyl-CoA Desaturase; FADS5; SCD1; Fatty Acid Desaturase; Acyl-CoA Desaturase; SCDOS; Stearoyl-CoA Desaturase (Delta-9-Desaturase); Stearoyl-CoA Desaturase Opposite Strand; Delta(9)-Desaturase; EC 1.14.19.1; HSCD1; Predicted Protein Of HQ0998; Delta-9-Desaturase; Delta-9 Desaturase; MSTP008
Background

Gene Name: SCD

NCBI Gene Entry: 6319

UniProt Entry: O00767

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 25-37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB215

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SCD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000). Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Stearoyl-CoA desaturase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SCD
  • Catalog：
    5218
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000). Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Stearoyl-CoA desaturase.
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