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CYP27A1 Recombinant Rabbit mAb

#5245

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Recombinant Antibody
Western blotting analysis using CYP27A1 antibody (Cat#5245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP27A1 antibody (Cat#5245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CYP27A1
  • Catalog:
    5245
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name CYP27A1 Recombinant Rabbit mAb
Aliases CYP27A1; Cytochrome P450 Family 27 Subfamily A Member 1; CYP27; CP27; CTX; Cytochrome P450, Subfamily XXVIIA (Steroid 27-Hydroxylase, Cerebrotendinous Xanthomatosis), Polypeptide 1; Cytochrome P450, Family 27, Subfamily A, Polypeptide 1; Sterol 26-Hydroxylase, Mitochondrial; Vitamin D(3) 25-Hydroxylase; Cytochrome P-450C27/25; Sterol 27-Hydroxylase; Cytochrome P450 27; 5-Beta-Cholestane-3-Alpha, 7-Alpha, 12-Alpha-Triol 26-Hydroxylase; 5-Beta-Cholestane-3-Alpha, 7-Alpha, 12-Alpha-Triol 27-Hydroxylase; 5-Beta-Cholestane-3-Alpha,7-Alpha,12-Alpha-Triol 26-Hydroxylase; Cholestanetriol 26-Monooxygenase; Cerebrotendinous Xanthomatosis; EC 1.14.15.15
Background

Gene Name: CYP27A1

NCBI Gene Entry: 1593

UniProt Entry: Q02318

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 53 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB330

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human CYP27A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP27A1 antibody (Cat#5245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP27A1 antibody (Cat#5245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CYP27A1 antibody (Cat#5245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP27A1 antibody (Cat#5245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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