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Home>Product>Primary Antibody

SERPING1 Mouse mAb

#5281

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Western blotting analysis using SERPING1 antibody (Cat#5281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPING1 antibody (Cat#5281, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with SERPING1 antibody (Cat#5281, 1:1,000). Nuclei were stained blue with DAPI; SERPING1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SERPING1 expression in C2C12 cells using SERPING1 antibody (Cat#5281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPING1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SERPING1
  • Catalog：
    5281
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SERPING1 Mouse mAb
Aliases SERPING1; Serpin Family G Member 1; C1IN; Plasma Protease C1 Inhibitor; C1INH; HAE1; HAE2; C1NH; Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1; C1 Esterase Inhibitor; C1-Inhibiting Factor; C1-Inhibitor; Serpin G1; C1-INH; Serine (Or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1, (Angioedema, Hereditary); Serine/Cysteine Proteinase Inhibitor Clade G Member 1; Serpin Peptidase Inhibitor Clade G Member 1; Epididymis Secretory Sperm Binding Protein; Complement Component 1 Inhibitor; Angioedema, Hereditary; C1 Inh; C1Inh
Background

Gene Name: SERPING1

NCBI Gene Entry: 710

UniProt Entry: P05155

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB730

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Purified C1 inhibitor from human blood
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SERPING1 antibody (Cat#5281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPING1 antibody (Cat#5281, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with SERPING1 antibody (Cat#5281, 1:1,000). Nuclei were stained blue with DAPI; SERPING1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SERPING1 expression in C2C12 cells using SERPING1 antibody (Cat#5281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPING1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SERPING1
  • Catalog：
    5281
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SERPING1 antibody (Cat#5281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPING1 antibody (Cat#5281, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with SERPING1 antibody (Cat#5281, 1:1,000). Nuclei were stained blue with DAPI; SERPING1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SERPING1 expression in C2C12 cells using SERPING1 antibody (Cat#5281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SERPING1.
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