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IL1R1 Mouse Oligoclonal Antibody

#5290

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Western blotting analysis using IL1R1 antibody (Cat#5290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL1R1 antibody (Cat#5290, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IL1R1 expression in A549 cells using IL1R1 antibody (Cat#5290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IL1R1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    IL1R1
  • Catalog:
    5290
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name IL1R1 Mouse Oligoclonal Antibody
Aliases IL1R1; Interleukin 1 Receptor Type 1; D2S1473; CD121A; IL1RA; IL1R; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Type 1; Interleukin-1 Receptor Type I; Interleukin-1 Receptor Alpha; IL-1R-Alpha; IL-1RT1; P80; Interleukin 1 Receptor Alpha, Type I; Interleukin-1 Receptor, Type I; Interleukin 1 Receptor, Type I; CD121a Antigen; EC 3.2.2.6; IL-1RT-1; IL-1R-1; IL1RT1; CRMO3
Background

Gene Name: IL1R1

NCBI Gene Entry: 3554

UniProt Entry: P14778

Application Information

Molecular Weight: Predicted, 65 kDa; observed, 50 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human IL1R1
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using IL1R1 antibody (Cat#5290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL1R1 antibody (Cat#5290, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of IL1R1 expression in A549 cells using IL1R1 antibody (Cat#5290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IL1R1.
Western blotting analysis using IL1R1 antibody (Cat#5290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL1R1 antibody (Cat#5290, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IL1R1 expression in A549 cells using IL1R1 antibody (Cat#5290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IL1R1.
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