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Home>Product>Primary Antibody

DYNC1LI1 Mouse mAb

#5405

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Western blotting analysis using DYNC1LI1 antibody (Cat#5405). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNC1LI1 antibody (Cat#5405, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with DYNC1LI1 antibody (Cat#5405, 1:1,000). Nuclei were stained blue with DAPI; DYNC1LI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DYNC1LI1 expression in HepG2 cells using DYNC1LI1 antibody (Cat#5405, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DYNC1LI1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DYNC1LI1
  • Catalog：
    5405
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name DYNC1LI1 Mouse mAb
Aliases DYNC1LI1; Dynein Cytoplasmic 1 Light Intermediate Chain 1; DNCLI1; Dynein, Cytoplasmic, Light Intermediate Polypeptide 1; Cytoplasmic Dynein 1 Light Intermediate Chain 1; Dynein Light Intermediate Chain 1, Cytosolic 3; Dynein Light Chain A; DLIC-1; DLC-A; LIC1
Background

Gene Name: DYNC1LI1

NCBI Gene Entry: 51143

UniProt Entry: Q9Y6G9

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 57 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1355

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human DYNC1LI1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DYNC1LI1 antibody (Cat#5405). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNC1LI1 antibody (Cat#5405, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with DYNC1LI1 antibody (Cat#5405, 1:1,000). Nuclei were stained blue with DAPI; DYNC1LI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DYNC1LI1 expression in HepG2 cells using DYNC1LI1 antibody (Cat#5405, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DYNC1LI1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DYNC1LI1
  • Catalog：
    5405
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DYNC1LI1 antibody (Cat#5405). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNC1LI1 antibody (Cat#5405, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with DYNC1LI1 antibody (Cat#5405, 1:1,000). Nuclei were stained blue with DAPI; DYNC1LI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DYNC1LI1 expression in HepG2 cells using DYNC1LI1 antibody (Cat#5405, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DYNC1LI1.
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