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FARSB Mouse mAb

#5415

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Western blotting analysis using FARSB antibody (Cat#5415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FARSB antibody (Cat#5415, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FARSB expression in HepG2 cells using FARSB antibody (Cat#5415, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FARSB.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FARSB
  • Catalog:
    5415
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name FARSB Mouse mAb
Aliases FARSB; Phenylalanyl-TRNA Synthetase Subunit Beta; FRSB; FARSLB; PheHB; Phenylalanyl-TRNA Synthetase-Like, Beta Subunit; Phenylalanine TRNA Ligase 1, Beta, Cytoplasmic; Phenylalanyl-TRNA Synthetase Beta Subunit; Phenylalanine--TRNA Ligase Beta Subunit; EC 6.1.1.20; PheRS; Phenylalanine-TRNA Synthetase-Like, Beta Subunit; Phenylalanyl-TRNA Synthetase Beta Chain; Phenylalanine-TRNA Ligase Beta Chain; EC 6.1.1; HSPC173; NEDBLLA; RILDBC1; RILDBC; RJBS
Background

Gene Name: FARSB

NCBI Gene Entry: 10056

UniProt Entry: Q9NSD9

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 66 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1405

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human FARSB
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using FARSB antibody (Cat#5415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FARSB antibody (Cat#5415, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of FARSB expression in HepG2 cells using FARSB antibody (Cat#5415, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FARSB.
Western blotting analysis using FARSB antibody (Cat#5415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FARSB antibody (Cat#5415, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FARSB expression in HepG2 cells using FARSB antibody (Cat#5415, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FARSB.
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