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Home>Product>Primary Antibody

CD3G Recombinant Rabbit mAb

#5444

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Recombinant Antibody
Western blotting analysis using CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of jurkat cells with CD3G antibody (Cat#5444, 1:1,000). Nuclei were stained blue with DAPI; CD3G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD3G expression in Jurkat cells using CD3G antibody (Cat#5444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD3G.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD3G
  • Catalog：
    5444
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name CD3G Recombinant Rabbit mAb
Aliases CD3G; CD3 Gamma Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Gamma Chain; CD3-GAMMA; CD3GAMMA; CD3g Antigen, Gamma Polypeptide (TiT3 Complex); CD3g Molecule, Gamma (CD3-TCR Complex); T-Cell Receptor T3 Gamma Chain; T3G; T-Cell Antigen Receptor Complex, Gamma Subunit Of T3; CD3g Molecule, Epsilon (CD3-TCR Complex); CD3g Molecule; CD3g Antigen; IMD17
Background

Gene Name: CD3G

NCBI Gene Entry: 917

UniProt Entry: P09693

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 18-28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1585

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CD3G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of jurkat cells with CD3G antibody (Cat#5444, 1:1,000). Nuclei were stained blue with DAPI; CD3G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CD3G expression in Jurkat cells using CD3G antibody (Cat#5444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD3G.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD3G
  • Catalog：
    5444
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of jurkat cells with CD3G antibody (Cat#5444, 1:1,000). Nuclei were stained blue with DAPI; CD3G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD3G expression in Jurkat cells using CD3G antibody (Cat#5444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD3G.
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