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Home>Product>Primary Antibody

POU2AF1 Recombinant Rabbit mAb

#5486

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Recombinant Antibody
Western blotting analysis using POU2AF1 antibody (Cat#5486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#5486, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#5486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POU2AF1
  • Catalog：
    5486
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name POU2AF1 Recombinant Rabbit mAb
Aliases POU2AF1; POU Class 2 Homeobox Associating Factor 1; OCA-B; OBF1; BOB1; OCT-Binding Factor 1; POU Domain Class 2-Associating Factor 1; B-Cell-Specific Coactivator OBF-1; POU Class 2 Associating Factor 1; BOB-1; POU Domain Class 2, Associating Factor 1; OBF-1; OCAB
Background

Gene Name: POU2AF1

NCBI Gene Entry: 5450

UniProt Entry: Q16633

Application Information

Molecular Weight: Predicted, 27 kDa; observed, 35-40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1715

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human POU2AF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using POU2AF1 antibody (Cat#5486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#5486, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#5486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POU2AF1
  • Catalog：
    5486
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using POU2AF1 antibody (Cat#5486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#5486, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#5486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
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