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STK17B Recombinant Rabbit mAb

#5513

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Recombinant Antibody
Western blotting analysis using STK17B antibody (Cat#5513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STK17B antibody (Cat#5513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with STK17B antibody (Cat#5513, 1:1,000). Nuclei were stained blue with DAPI; STK17B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STK17B expression in HepG2 cells using STK17B antibody (Cat#5513, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STK17B.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    STK17B
  • Catalog:
    5513
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M
Information
Product Name STK17B Recombinant Rabbit mAb
Aliases STK17B; Serine/Threonine Kinase 17b; DRAK2; DAP Kinase-Related Apoptosis-Inducing Protein Kinase 2; Death-Associated Protein Kinase-Related 2; Serine/Threonine-Protein Kinase 17B; EC 2.7.11.1; Serine/Threonine Kinase 17b (Apoptosis-Inducing); EC 2.7.11
Background

Gene Name: STK17B

NCBI Gene Entry: 9262

UniProt Entry: O94768

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1845

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human DRAK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using STK17B antibody (Cat#5513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STK17B antibody (Cat#5513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with STK17B antibody (Cat#5513, 1:1,000). Nuclei were stained blue with DAPI; STK17B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of STK17B expression in HepG2 cells using STK17B antibody (Cat#5513, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STK17B.
Western blotting analysis using STK17B antibody (Cat#5513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STK17B antibody (Cat#5513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with STK17B antibody (Cat#5513, 1:1,000). Nuclei were stained blue with DAPI; STK17B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STK17B expression in HepG2 cells using STK17B antibody (Cat#5513, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STK17B.
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