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GNB3 Recombinant Rabbit mAb

#5574

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Recombinant Antibody
Western blotting analysis using GNB3 antibody (Cat#5574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GNB3 antibody (Cat#5574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of GNB3 expression in HepG2 cells using GNB3 antibody (Cat#5574, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GNB3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    GNB3
  • Catalog:
    5574
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name GNB3 Recombinant Rabbit mAb
Aliases GNB3; G Protein Subunit Beta 3; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Subunit Beta-3; Transducin Beta Chain 3; Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 3; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Beta Subunit 3; Heterotrimeric Guanine Nucleotide-Binding Protein 2D; GTP-Binding Regulatory Protein Beta-3 Chain; Hypertension Associated Protein; G Protein, Beta-3 Subunit; CSNB1H; HG2D
Background

Gene Name: GNB3

NCBI Gene Entry: 2784

UniProt Entry: P16520

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4330

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human GNB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using GNB3 antibody (Cat#5574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GNB3 antibody (Cat#5574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of GNB3 expression in HepG2 cells using GNB3 antibody (Cat#5574, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GNB3.
Western blotting analysis using GNB3 antibody (Cat#5574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GNB3 antibody (Cat#5574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of GNB3 expression in HepG2 cells using GNB3 antibody (Cat#5574, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GNB3.
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