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Home>Product>Primary Antibody

NDUFS6 Recombinant Rabbit mAb

#5583

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Recombinant Antibody
Western blotting analysis using NDUFS6 antibody (Cat#5583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS6 antibody (Cat#5583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of A549 cells with NDUFS6 antibody(Cat#5583, 1:1,000). Nuclei were stained blue with DAPI; NDUFS6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFS6 expression in A549 cells using NDUFS6 antibody (Cat#5583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFS6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFS6
  • Catalog：
    5583
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name NDUFS6 Recombinant Rabbit mAb
Aliases NDUFS6; NADH:Ubiquinone Oxidoreductase Subunit S6; NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 6, Mitochondrial; CI-13kA; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 6, 13kDa (NADH-Coenzyme Q Reductase); NADH-Ubiquinone Oxidoreductase 13 KDa-A Subunit; Complex I 13kDa Subunit A; CI-13kD-A; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 6 (13kD) (NADH-Coenzyme Q Reductase); Complex I, Mitochondrial Respiratory Chain, 13-KD Subunit; NADH:Ubiquinone Oxidoreductase NDUFS6 Subunit; Complex I-13kD-A; CI13KDA; MC1DN9
Background

Gene Name: NDUFS6

NCBI Gene Entry: 4726

UniProt Entry: O75380

Application Information

Molecular Weight: Predicted, 14 kDa; observed, 14 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4375

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NDUFS6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFS6 antibody (Cat#5583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS6 antibody (Cat#5583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of A549 cells with NDUFS6 antibody(Cat#5583, 1:1,000). Nuclei were stained blue with DAPI; NDUFS6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NDUFS6 expression in A549 cells using NDUFS6 antibody (Cat#5583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFS6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFS6
  • Catalog：
    5583
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NDUFS6 antibody (Cat#5583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS6 antibody (Cat#5583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of A549 cells with NDUFS6 antibody(Cat#5583, 1:1,000). Nuclei were stained blue with DAPI; NDUFS6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFS6 expression in A549 cells using NDUFS6 antibody (Cat#5583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFS6.
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