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Home>Product>Primary Antibody

Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit mAb

#5588

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Recombinant Antibody
Western blotting analysis using phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of PC-3 cells with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) expression in PC-3 cells using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)C antibody (Cat#5588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PAK1/2/3 (Ser144/Ser141/Ser154).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAK3
  • Catalog：
    5588
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit mAb
Aliases PAK3; P21 (RAC1) Activated Kinase 3; BPAK; P21 Protein (Cdc42/Rac)-Activated Kinase 3; Serine/Threonine-Protein Kinase PAK 3; P21 (CDKN1A)-Activated Kinase 3; Oligophrenin-3; EC 2.7.11.1; Beta-PAK; HPAK3; MRX30; MRX47; OPHN3; PAK-3; Adriamycin Resistance-Associated; Mental Retardation, X-Linked; P21-Activated Kinase 3; EC 2.7.11; PAK3beta; XLID30; ARA
Background

Gene Name: PAK3

NCBI Gene Entry: 5063

UniProt Entry: O75914

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4400

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of PC-3 cells with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) expression in PC-3 cells using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)C antibody (Cat#5588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PAK1/2/3 (Ser144/Ser141/Ser154).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAK3
  • Catalog：
    5588
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of PC-3 cells with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody (Cat#5588, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) expression in PC-3 cells using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)C antibody (Cat#5588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PAK1/2/3 (Ser144/Ser141/Ser154).
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