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DAPK3 Recombinant Rabbit mAb

#5669

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Recombinant Antibody
Western blotting analysis using DAPK3 antibody (Cat#5669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAPK3 antibody (Cat#5669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DAPK3 expression in HT-1080 cells using DAPK3 antibody (Cat#5669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DAPK3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    DAPK3
  • Catalog:
    5669
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name DAPK3 Recombinant Rabbit mAb
Aliases DAPK3; Death Associated Protein Kinase 3; ZIPK; ZIP; Death-Associated Protein Kinase 3; Zipper-Interacting Protein Kinase; DAP-Like Kinase; DAP Kinase 3; MYPT1 Kinase; EC 2.7.11.1; ZIP-Kinase; EC 2.7.11; DLK; Dlk
Background

Gene Name: DAPK3

NCBI Gene Entry: 1613

UniProt Entry: O43293

Application Information

Molecular Weight: Predicted, 53 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4795

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human DAPK3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DAPK3 antibody (Cat#5669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAPK3 antibody (Cat#5669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of DAPK3 expression in HT-1080 cells using DAPK3 antibody (Cat#5669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DAPK3.
Western blotting analysis using DAPK3 antibody (Cat#5669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAPK3 antibody (Cat#5669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DAPK3 expression in HT-1080 cells using DAPK3 antibody (Cat#5669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DAPK3.
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