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Home>Product>Primary Antibody

NDUFB10 Mouse mAb

#5705

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Western blotting analysis using NDUFB10 antibody (Cat#5705). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#5705, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NDUFB10 antibody (Cat#5705, 1:1,000). Nuclei were stained blue with DAPI; NDUFB10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFB10 expression in HT-1080 cells using NDUFB10 antibody (Cat#5705, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NDUFB10.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFB10
  • Catalog：
    5705
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NDUFB10 Mouse mAb
Aliases NDUFB10; NADH:Ubiquinone Oxidoreductase Subunit B10; PDSW; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 10, 22kDa; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 10; NADH-Ubiquinone Oxidoreductase PDSW Subunit; Complex I PDSW Subunit; Complex I-PDSW; CI-PDSW; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 10 (22kD, PDSW); NADH Ubiquinone Oxidoreductase PDSW Subunit (RH 16p13.3); MC1DN35
Background

Gene Name: NDUFB10

NCBI Gene Entry: 4716

UniProt Entry: O96000

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4990

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NDUFB10
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFB10 antibody (Cat#5705). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#5705, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with NDUFB10 antibody (Cat#5705, 1:1,000). Nuclei were stained blue with DAPI; NDUFB10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NDUFB10 expression in HT-1080 cells using NDUFB10 antibody (Cat#5705, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NDUFB10.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFB10
  • Catalog：
    5705
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NDUFB10 antibody (Cat#5705). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB10 antibody (Cat#5705, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NDUFB10 antibody (Cat#5705, 1:1,000). Nuclei were stained blue with DAPI; NDUFB10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFB10 expression in HT-1080 cells using NDUFB10 antibody (Cat#5705, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NDUFB10.
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