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RPL10 Mouse mAb

#5921

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Western blotting analysis using ribosomal protein L 10 antibody (Cat#5921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein L 10 antibody (Cat#5921, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Ribosomal protein L10 antibody (Cat#5921, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein L10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ribosomal protein L10 expression in C2C12 cells using Ribosomal protein L10 antibody (Cat#5921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ribosomal protein L10.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RPL10
  • Catalog:
    5921
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name RPL10 Mouse mAb
Aliases RPL10; Ribosomal Protein L10; DXS648E; QM; DXS648; UL16; NOV; L10; Large Ribosomal Subunit Protein UL16; 60S Ribosomal Protein L10; Laminin Receptor Homolog; Tumor Suppressor QM; FLJ23544; Wilms Tumor-Related Protein; Protein QM; AUTSX5; MRXS35
Background

Gene Name: RPL10

NCBI Gene Entry: 6134

UniProt Entry: P27635

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5840

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human RPL10
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ribosomal protein L 10 antibody (Cat#5921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein L 10 antibody (Cat#5921, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Ribosomal protein L10 antibody (Cat#5921, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein L10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Ribosomal protein L10 expression in C2C12 cells using Ribosomal protein L10 antibody (Cat#5921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ribosomal protein L10.
Western blotting analysis using ribosomal protein L 10 antibody (Cat#5921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein L 10 antibody (Cat#5921, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Ribosomal protein L10 antibody (Cat#5921, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein L10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ribosomal protein L10 expression in C2C12 cells using Ribosomal protein L10 antibody (Cat#5921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ribosomal protein L10.
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