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Home>Product>Primary Antibody

KD-Validated AGTR1 Recombinant Rabbit mAb

#61173

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild-type (WT) and AGTR1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild type (WT) and AGTR1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AGTR1 in HAP-1 cells using AGTR1 antibody (Cat#61173, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AGTR1
  • Catalog：
    61173
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated AGTR1 Recombinant Rabbit mAb
Aliases AGTR1; Angiotensin II Receptor Type 1; AT2R1; AGTR1A; AT2R1B; AGTR1B; HAT1R; AG2S; AT1B; AT1; Type-1 Angiotensin II Receptor; AT1 Receptor; AT2R1A; AT1AR; AT1BR; ATR1; Type-1B Angiotensin II Receptor; Angiotensin II Type 1 Receptor; Angiotensin II Type-1 Receptor; Angiotensin Receptor 1B; AT1R
Background

Gene Name: AGTR1

NCBI Gene Entry: 185

UniProt Entry: P30556

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 41 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6060

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human AGTR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild-type (WT) and AGTR1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild type (WT) and AGTR1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of AGTR1 in HAP-1 cells using AGTR1 antibody (Cat#61173, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AGTR1
  • Catalog：
    61173
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild-type (WT) and AGTR1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using AGTR1 antibody (Cat#61173). AGTR1 expression in wild type (WT) and AGTR1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AGTR1 antibody (Cat#61173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AGTR1 in HAP-1 cells using AGTR1 antibody (Cat#61173, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
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