Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, inhibin subunit beta B.