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KD-Validated Inhibin Subunit Beta B Recombinant Rabbit mAb

#61184

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, inhibin subunit beta B.
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    INHBB
  • Catalog:
    61184
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M
Information
Product Name KD-Validated Inhibin Subunit Beta B Recombinant Rabbit mAb
Aliases INHBB; Inhibin Subunit Beta B; Inhibin Beta B Chain; Activin Beta-B Chain; Inhibin, Beta B (Activin AB Beta Polypeptide); Activin AB Beta Polypeptide; Inhibin Beta B Subunit; Inhibin, Beta B; Inhibin, Beta-2
Background

Gene Name: INHBB

NCBI Gene Entry: 3625

UniProt Entry: P09529

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6100

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Inhibin beta B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, inhibin subunit beta B.
Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, inhibin subunit beta B.
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