Western blotting analysis using H2AX antibody (Cat#6124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with H2AX antibody (Cat#6124, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with H2AX antibody (Cat#6124,1:1,000). Nuclei were stained blue with DAPI; H2AX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Applications Tested: Western Blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human H2AF
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using H2AX antibody (Cat#6124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with H2AX antibody (Cat#6124, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with H2AX antibody (Cat#6124,1:1,000). Nuclei were stained blue with DAPI; H2AX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using H2AX antibody (Cat#6124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with H2AX antibody (Cat#6124, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with H2AX antibody (Cat#6124,1:1,000). Nuclei were stained blue with DAPI; H2AX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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