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Home>Product>Primary Antibody

KD-Validated FZD8 Recombinant Rabbit mAb

#61378

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using FZD8 antibody (Cat#61378). FZD8 expression in wild type (WT) and FZD8 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FZD8 antibody (Cat#61378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with FZD8 antibody (Cat#61378, 1:1,000). Nuclei were stained blue with DAPI; FZD8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FZD8 expression in HepG2 cells using FZD8 antibody (Cat#61378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FZD8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FZD8
  • Catalog：
    61378
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated FZD8 Recombinant Rabbit mAb
Aliases FZD8; Frizzled Class Receptor 8; Frizzled 8, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 8; Frizzled-8; Frizzled (Drosophila) Homolog 8; Frizzled Homolog 8 (Drosophila); Frizzled Homolog 8; FZ-8; HFZ8; Fz-8; HFz8
Background

Gene Name: FZD8

NCBI Gene Entry: 8325

UniProt Entry: Q9H461

Application Information

Molecular Weight: Predicted, 73 kDa; observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2395

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human FZD8
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using FZD8 antibody (Cat#61378). FZD8 expression in wild type (WT) and FZD8 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FZD8 antibody (Cat#61378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with FZD8 antibody (Cat#61378, 1:1,000). Nuclei were stained blue with DAPI; FZD8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of FZD8 expression in HepG2 cells using FZD8 antibody (Cat#61378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FZD8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FZD8
  • Catalog：
    61378
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using FZD8 antibody (Cat#61378). FZD8 expression in wild type (WT) and FZD8 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FZD8 antibody (Cat#61378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with FZD8 antibody (Cat#61378, 1:1,000). Nuclei were stained blue with DAPI; FZD8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FZD8 expression in HepG2 cells using FZD8 antibody (Cat#61378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FZD8.
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