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Home>Product>Primary Antibody

KD-Validated Ribosomal Protein S6 Kinase B1 Recombinant Rabbit mAb

#61477

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using RPS6KB1 antibody (Cat#61477). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RPS6KB1 antibody (Cat#61477). S6K1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with RPS6KB1 antibody (Cat#61477, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of RPS6KB1 expression in C2C12 cells using RPS6KB1 antibody (Cat#61477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RPS6KB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    61477
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Ribosomal Protein S6 Kinase B1 Recombinant Rabbit mAb
Aliases RPS6KB1; Ribosomal Protein S6 Kinase B1; S6K1; P70(S6K)-Alpha; STK14A; PS6K; S6K; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase Beta-1; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Serine/Threonine Kinase 14 Alpha; P70 Ribosomal S6 Kinase Alpha; P70 S6 Kinase Alpha; P70 S6K-Alpha; P70-Alpha; P70-S6K 1; EC 2.7.11; P70-S6K; P70S6K1
Background

Gene Name: RPS6KB1

NCBI Gene Entry: 6198

UniProt Entry: P23443

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 60-75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2615

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human S6K1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RPS6KB1 antibody (Cat#61477). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using RPS6KB1 antibody (Cat#61477). S6K1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9C2 cells with RPS6KB1 antibody (Cat#61477, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of RPS6KB1 expression in C2C12 cells using RPS6KB1 antibody (Cat#61477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RPS6KB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    61477
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RPS6KB1 antibody (Cat#61477). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RPS6KB1 antibody (Cat#61477). S6K1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with RPS6KB1 antibody (Cat#61477, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of RPS6KB1 expression in C2C12 cells using RPS6KB1 antibody (Cat#61477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RPS6KB1.
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