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ALDH2 Mouse mAb

#6149

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Western blotting analysis using ALDH2 antibody (Cat#6149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH2 antibody (Cat#6149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ALDH2 antibody (Cat#6149, 1:1,000). Nuclei were stained blue with DAPI; ALDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ALDH2 expression in C2C12 cells using ALDH2 antibody (Cat#6149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALDH2.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    ALDH2
  • Catalog:
    6149
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name ALDH2 Mouse mAb
Aliases ALDH2; Aldehyde Dehydrogenase 2 Family Member; Aldehyde Dehydrogenase 2 Family (Mitochondrial); Aldehyde Dehydrogenase, Mitochondrial; ALDH Class; EC 1.2.1.3; ALDH-E2; ALDHI; ALDM; Nucleus-Encoded Mitochondrial Aldehyde Dehydrogenase; Epididymis Secretory Sperm Binding Protein; Acetaldehyde Dehydrogenase; Liver Mitochondrial ALDH; EC 1.2.1
Background

Gene Name: ALDH2

NCBI Gene Entry: 217

UniProt Entry: P05091

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB7300

Species Reactivity: Human, mouse, rat

Applications Tested: Western Blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human ALDH2
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ALDH2 antibody (Cat#6149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH2 antibody (Cat#6149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with ALDH2 antibody (Cat#6149, 1:1,000). Nuclei were stained blue with DAPI; ALDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ALDH2 expression in C2C12 cells using ALDH2 antibody (Cat#6149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALDH2.
Western blotting analysis using ALDH2 antibody (Cat#6149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH2 antibody (Cat#6149, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ALDH2 antibody (Cat#6149, 1:1,000). Nuclei were stained blue with DAPI; ALDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ALDH2 expression in C2C12 cells using ALDH2 antibody (Cat#6149, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALDH2.
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