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Home>Product>Primary Antibody

KD-Validated AKR1C1/AKR1C2 Recombinant Rabbit mAb

#61575

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C1 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C2 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with AKR1C1/AKR1C2 antibody (Cat#61575, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using AKR1C1 / AKR1C2 antibody (Cat#61575, 1:1,000), Top panel: wild-type (WT); Bottom panal: AKR1C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AKR1C1 / AKR1C2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using AKR1C1/AKR1C2 antibody (Cat#61575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKR1C1/AKR1C2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKR1C1/AKR1C2
  • Catalog：
    61575
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated AKR1C1/AKR1C2 Recombinant Rabbit mAb
Aliases AKR1C1/2; Aldo-Keto Reductase Family 1 Member C1/2; DDH; DD1/2; HAKRC; MBAB; DDH1/2; Dihydrodiol Dehydrogenase 1/2; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase; High-Affinity Hepatic Bile Acid-Binding Protein; Chlordecone Reductase Homolog HAKRC; Dihydrodiol Dehydrogenase 1/2; HBAB; Aldo-Keto Reductase Family 1, Member C1/2 (Dihydrodiol Dehydrogenase 1; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase); Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; Type II 3-Alpha-Hydroxysteroid Dehydrogenase; 20 Alpha-Hydroxysteroid Dehydrogenase; 20-Alpha-Hydroxysteroid Dehydrogenase; Hepatic Dihydrodiol Dehydrogenase; Dihydrodiol Dehydrogenase 1/2; Aldo-Keto Reductase C; Indanol Dehydrogenase; 20-ALPHA-HSD; 20-Alpha-HSD; EC 1.1.1.112; EC 1.1.1.209; EC 1.1.1.210; EC 1.1.1.357; EC 1.1.1.149; 2-ALPHA-HSD; EC 1.1.1.51; EC 1.1.1.53; EC 1.1.1.62; EC 1.3.1.20; EC 1.1.1.; EC 1.1.1; DD1/DD2; H-37; C9
Background

Gene Name: AKR1C1/AKR1C2

NCBI Gene Entry: 1645 , 1646

UniProt Entry: Q04828 , P52895

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3175

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthetic peptide of human AKR1C1 / AKR1C2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C1 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C2 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with AKR1C1/AKR1C2 antibody (Cat#61575, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using AKR1C1 / AKR1C2 antibody (Cat#61575, 1:1,000), Top panel: wild-type (WT); Bottom panal: AKR1C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AKR1C1 / AKR1C2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using AKR1C1/AKR1C2 antibody (Cat#61575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKR1C1/AKR1C2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKR1C1/AKR1C2
  • Catalog：
    61575
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C1 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKR1C1/AKR1C2 antibody (Cat#61575). AKR1C1/AKR1C2 expression in wild type (WT) and AKR1C2 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1/AKR1C2 antibody (Cat#61575, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with AKR1C1/AKR1C2 antibody (Cat#61575, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using AKR1C1 / AKR1C2 antibody (Cat#61575, 1:1,000), Top panel: wild-type (WT); Bottom panal: AKR1C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AKR1C1 / AKR1C2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using AKR1C1/AKR1C2 antibody (Cat#61575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKR1C1/AKR1C2.
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