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Home>Product>Primary Antibody

KD-Validated NEDD4L Recombinant Rabbit mAb

#61581

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using NEDD4L antibody (Cat#61581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NEDD4L antibody (Cat#61581). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NEDD4L expression in HepG2 cells using NEDD4L antibody (Cat#61581, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NEDD4L.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEDD4L
  • Catalog：
    61581
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated NEDD4L Recombinant Rabbit mAb
Aliases NEDD4L; NEDD4 Like E3 Ubiquitin Protein Ligase; KIAA0439; NEDD4-2; RSP5; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like, E3 Ubiquitin Protein Ligase; HECT-Type E3 Ubiquitin Transferase NED4L; E3 Ubiquitin-Protein Ligase NEDD4-Like; NEDD4.2; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like; Ubiquitin-Protein Ligase Rsp5; EC 2.3.2.26;
EC 2.3.2.36; HNEDD4-2; EC 6.3.2; Nedd4-2; PVNH7; NEDL3
Background

Gene Name: NEDD4L

NCBI Gene Entry: 23327

UniProt Entry: Q96PU5

Application Information

Molecular Weight: Predicted, 112 kDa; observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2910

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human NEDD4-2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using NEDD4L antibody (Cat#61581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using NEDD4L antibody (Cat#61581). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of NEDD4L expression in HepG2 cells using NEDD4L antibody (Cat#61581, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NEDD4L.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEDD4L
  • Catalog：
    61581
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NEDD4L antibody (Cat#61581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NEDD4L antibody (Cat#61581). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NEDD4L expression in HepG2 cells using NEDD4L antibody (Cat#61581, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NEDD4L.
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