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Home>Product>Primary Antibody

KD-Validated BAZ1B Recombinant Rabbit mAb

#61613

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using BAZ1B antibody (Cat#61613). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BAZ1B antibody (Cat#61613). BAZ1B expression in wild type (WT) and BAZ1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse colon tissue using BAZ1B antibody (Cat#61613, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of Hela cells with BAZ1B antibody (Cat#61613, 1:1,000). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using BAZ1B antibody (Cat#61613, 1:1,000), Top panel: wild-type (WT); Bottom panal: BAZ1B shRNA knockdown (KD). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of BAZ1B expression in HeLa cells using BAZ1B antibody (Cat#61613, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BAZ1B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    BAZ1B
  • Catalog：
    61613
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated BAZ1B Recombinant Rabbit mAb
Aliases BAZ1B; Bromodomain Adjacent To Zinc Finger Domain 1B; WSTF; WBSCR10; WBSCR9; Williams-Beuren Syndrome Chromosomal Region 10 Protein; Williams-Beuren Syndrome Chromosomal Region 9 Protein; Williams Syndrome; Transcription Factor; Tyrosine-Protein Kinase BAZ1B; Transcription Factor; WSTF; EC 2.7.10.2; HWALp2; Bromodomain Adjacent To Zinc Finger Domain Protein 1B; Williams-Beuren Syndrome Chromosome Region 10; Williams-Beuren Syndrome Chromosome Region 9; WBSC10
Background

Gene Name: BAZ1B

NCBI Gene Entry: 9031

UniProt Entry: Q9UIG0

Application Information

Molecular Weight: Predicted, 170 kDa; observed, 185 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3685

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human WSTF
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using BAZ1B antibody (Cat#61613). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using BAZ1B antibody (Cat#61613). BAZ1B expression in wild type (WT) and BAZ1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse colon tissue using BAZ1B antibody (Cat#61613, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of Hela cells with BAZ1B antibody (Cat#61613, 1:1,000). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using BAZ1B antibody (Cat#61613, 1:1,000), Top panel: wild-type (WT); Bottom panal: BAZ1B shRNA knockdown (KD). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of BAZ1B expression in HeLa cells using BAZ1B antibody (Cat#61613, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BAZ1B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BAZ1B
  • Catalog：
    61613
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using BAZ1B antibody (Cat#61613). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BAZ1B antibody (Cat#61613). BAZ1B expression in wild type (WT) and BAZ1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAZ1B antibody (Cat#61613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse colon tissue using BAZ1B antibody (Cat#61613, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of Hela cells with BAZ1B antibody (Cat#61613, 1:1,000). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using BAZ1B antibody (Cat#61613, 1:1,000), Top panel: wild-type (WT); Bottom panal: BAZ1B shRNA knockdown (KD). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of BAZ1B expression in HeLa cells using BAZ1B antibody (Cat#61613, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BAZ1B.
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