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KD-Validated Fc Gamma Receptor IIIa Recombinant Rabbit mAb

#61652

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using FCGR3A antibody (Cat#61652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FCGR3A antibody (Cat#61652). FCGR3A expression in wild-type (WT) and FCGR3A knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FCGR3A expression in HT-1080 cells using FCGR3A antibody (Cat#61652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FCGR3A.
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Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FCGR3A
  • Catalog:
    61652
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated Fc Gamma Receptor IIIa Recombinant Rabbit mAb
Aliases FCGR3A; Fc Gamma Receptor IIIa; FcgammaRIIIa; FcGRIIIA; CD16a; FCGR3; CD16; FCG3; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; Fc Fragment Of IgG, Low Affinity IIIa, Receptor (CD16a); Fc Fragment Of IgG Receptor IIIa; IgG Fc Receptor III-A; IgG Fc Receptor III-2; Fc-Gamma RIII-Alpha; CD16a Antigen; CD16-II; IGFR3; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A/III-B Recombinant; Low Affinity Immunoglobulin Gamma Receptor III-A Fc Fragment; Fc Fragment Of IgG, Low Affinity IIIa, Receptor For (CD16); Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B; Fc Fragment Of IgG, Low Affinity III, Receptor For (CD16); Immunoglobulin G Fc Receptor III; Fc-Gamma Receptor III-2 (CD 16); Neutrophil-Specific Antigen NA; Fc-Gamma Receptor IIIb (CD16); Fc Gamma Receptor III-A; Fc-Gamma RIIIa; Fc-Gamma RIII; FcgammaRIIIA; FCGRIII; FCRIIIA; FcRIIIa; FCR-10; FCRIII; FcRIII; FcR-10; IMD20; CD16A
Background

Gene Name: FCGR3A

NCBI Gene Entry: 2214

UniProt Entry: P08637

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3150

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CD16
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using FCGR3A antibody (Cat#61652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using FCGR3A antibody (Cat#61652). FCGR3A expression in wild-type (WT) and FCGR3A knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of FCGR3A expression in HT-1080 cells using FCGR3A antibody (Cat#61652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FCGR3A.
Western blotting analysis using FCGR3A antibody (Cat#61652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FCGR3A antibody (Cat#61652). FCGR3A expression in wild-type (WT) and FCGR3A knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FCGR3A antibody (Cat#61652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FCGR3A expression in HT-1080 cells using FCGR3A antibody (Cat#61652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FCGR3A.
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