KD-Validated PPP2R5D Recombinant Rabbit mAb - Product

KD-Validated PPP2R5D Recombinant Rabbit mAb

#61681

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using PPP2R5D antibody (Cat#61681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using PPP2R5D antibody (Cat#61681). PPP2R5D expression in wild type (WT) and PPP2R5D knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of PPP2R5D expression in HepG2 cells using PPP2R5D antibody (Cat#61681, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PPP2R5D.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP2R5D
Catalog：

61681
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated PPP2R5D Recombinant Rabbit mAb
Aliases	PPP2R5D; Protein Phosphatase 2 Regulatory Subunit B'Delta; Serine/Threonine-Protein Phosphatase 2A 56 KDa Regulatory Subunit Delta Isoform; B56delta; B56D; Protein Phosphatase 2, Regulatory Subunit B (B56), Delta Isoform; PP2A B Subunit Isoform PR61-Delta; PP2A B Subunit Isoform B56-Delta; PP2A B Subunit Isoform B'-Delta; PP2A B Subunit Isoform R5-Delta; MRD35; HJS1
Background	Gene Name: PPP2R5D NCBI Gene Entry: 5528 UniProt Entry: Q14738
Application Information	Molecular Weight: Predicted, 70 kDa; observed, 60-70 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3315 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human PPP2R5D
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using PPP2R5D antibody (Cat#61681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PPP2R5D antibody (Cat#61681). PPP2R5D expression in wild type (WT) and PPP2R5D knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PPP2R5D expression in HepG2 cells using PPP2R5D antibody (Cat#61681, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PPP2R5D.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP2R5D
Catalog：

61681
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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