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Home>Product>Primary Antibody

KD-Validated Caspase 9 Recombinant Rabbit mAb

#61769

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Caspase 9 antibody (Cat#61769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Caspase 9 antibody (Cat#61769). Caspase 9 expression in wild type (WT) and Caspase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with caspase 9 antibody (Cat#61769, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Caspase 9 antibody (Cat#61769, 1:1,000), Top panel: wild-type (WT); Bottom panal: Caspase 9 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Caspase 9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#61769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Caspase 9.
Validation of Caspase 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Caspase 9 antibody (Cat#61769, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    61769
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated Caspase 9 Recombinant Rabbit mAb
Aliases CASP9; Caspase 9; Apoptosis-Related Cysteine Peptidase; Regulatory Subunit 56; Apoptotic Protease Mch-6; PPP1R56; APAF-3; Apoptotic Protease Activating Factor 3; ICE-LAP6; caspase-9; MCH6; Protein Phosphatase 1; Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; CASP-9; APAF3; EC 3.4.22.62; Caspase 9; Apoptosis-Related Cysteine Protease; Apoptotic Protease-Activating Factor 3; Protein Phosphatase 1
Background

Gene Name: CASP9

NCBI Gene Entry: 842

UniProt Entry: P55211

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1005

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human Caspase-9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Caspase 9 antibody (Cat#61769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Caspase 9 antibody (Cat#61769). Caspase 9 expression in wild type (WT) and Caspase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with caspase 9 antibody (Cat#61769, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using Caspase 9 antibody (Cat#61769, 1:1,000), Top panel: wild-type (WT); Bottom panal: Caspase 9 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Caspase 9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
  • Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#61769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Caspase 9.
  • Validation of Caspase 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Caspase 9 antibody (Cat#61769, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    61769
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Caspase 9 antibody (Cat#61769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Caspase 9 antibody (Cat#61769). Caspase 9 expression in wild type (WT) and Caspase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Caspase 9 antibody (Cat#61769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with caspase 9 antibody (Cat#61769, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Caspase 9 antibody (Cat#61769, 1:1,000), Top panel: wild-type (WT); Bottom panal: Caspase 9 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Caspase 9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#61769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Caspase 9.
Validation of Caspase 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Caspase 9 antibody (Cat#61769, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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