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Home>Product>Primary Antibody

KD-Validated TRIP13 Mouse mAb

#61794

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Knockdown Validated
Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Thyroid hormone receptor interactor 13 expression in wild-type (WT) and thyroid hormone receptor interactor 13 (TRIP13) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Thyroid hormone receptor interactor 13 expression in HAP-1 cells using Thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Thyroid hormone receptor interactor 13.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TRIP13
  • Catalog：
    61794
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated TRIP13 Mouse mAb
Aliases TRIP13; Thyroid Hormone Receptor Interactor 13; 16E1BP; Human Papillomavirus Type 16 E1 Protein-Binding Protein; Thyroid Receptor-Interacting Protein 13; Pachytene Checkpoint Protein 2 Homolog; TR-Interacting Protein 13; 16E1-BP; TRIP-13; Thyroid Receptor Interacting Protein 13; HPV16 E1 Protein Binding Protein; HPV16 E1 Protein-Binding Protein; SH3TC1/TRIP13 Fusion; OZEMA9; OOMD9; MVA3; PCH2
Background

Gene Name: TRIP13

NCBI Gene Entry: 9319

UniProt Entry: Q15645

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 46 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3785

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human TRIP13
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Thyroid hormone receptor interactor 13 expression in wild-type (WT) and thyroid hormone receptor interactor 13 (TRIP13) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Thyroid hormone receptor interactor 13 expression in HAP-1 cells using Thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Thyroid hormone receptor interactor 13.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TRIP13
  • Catalog：
    61794
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using thyroid hormone receptor interactor 13 antibody (Cat#61794). Thyroid hormone receptor interactor 13 expression in wild-type (WT) and thyroid hormone receptor interactor 13 (TRIP13) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Thyroid hormone receptor interactor 13 expression in HAP-1 cells using Thyroid hormone receptor interactor 13 antibody (Cat#61794, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Thyroid hormone receptor interactor 13.
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