Western blotting analysis using ERK1/2 antibody (Cat#61797). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ERK1/2 antibody (Cat#61797). ERK1/2 expression in wild type (WT) and ERK1/2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ERK1/2 antibody (Cat #61797) antibody. Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Immunocytochemical staining of C2C12 cells with ERK1/2 antibody (Cat#61797, 1:1,000). Nuclei were stained blue with DAPI; ERK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ERK1/2 expression in C2C12 cells using ERK1/2 antibody (Cat#61797, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ERK1/2.
