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Home>Product>Primary Antibody

KD-Validated ROS1 Mouse mAb

#61882

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shRNA Knockdown Validated
Western blotting analysis using ROS1 antibody (Cat#61882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROS1 antibody (Cat#61882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ROS1 antibody (Cat#61882). ROS1 expression in wild type (WT) and ROS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROS1 antibody (Cat#61882, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ROS1 expression in HAP-1 cells using ROS1 antibody (Cat#61882, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ROS1.
Validation of ROS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROS1 antibody (Cat#61882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ROS1
  • Catalog：
    61882
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated ROS1 Mouse mAb
Aliases ROS1; ROS Proto-Oncogene 1, Receptor Tyrosine Kinase; MCF3; ROS; C-Ros-1; V-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; C-Ros Oncogene 1 , Receptor Tyrosine Kinase; Proto-Oncogene Tyrosine-Protein Kinase ROS; Proto-Oncogene C-Ros-1; V-Ros UR2 Sarcoma Virus Oncogene Homolog 1 (Avian); ROS Proto-Oncogene 1 , Receptor Tyrosine Kinase; Transmembrane Tyrosine-Specific Protein Kinase; Receptor Tyrosine Kinase C-Ros Oncogene 1; C-Ros Receptor Tyrosine Kinase; Proto-Oncogene C-Ros; EC 2.7.10.1
Background

Gene Name: ROS1

NCBI Gene Entry: 6098

UniProt Entry: P08922

Application Information

Molecular Weight: Predicted, 264 kDa; observed, 264 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4065

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human ROS1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ROS1 antibody (Cat#61882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROS1 antibody (Cat#61882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ROS1 antibody (Cat#61882). ROS1 expression in wild type (WT) and ROS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROS1 antibody (Cat#61882, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of ROS1 expression in HAP-1 cells using ROS1 antibody (Cat#61882, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ROS1.
  • Validation of ROS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROS1 antibody (Cat#61882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ROS1
  • Catalog：
    61882
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ROS1 antibody (Cat#61882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROS1 antibody (Cat#61882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ROS1 antibody (Cat#61882). ROS1 expression in wild type (WT) and ROS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROS1 antibody (Cat#61882, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ROS1 expression in HAP-1 cells using ROS1 antibody (Cat#61882, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ROS1.
Validation of ROS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROS1 antibody (Cat#61882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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