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Home>Product>Primary Antibody

KD-Validated WTAP Mouse mAb

#61884

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shRNA Knockdown Validated
Western blotting analysis using WTAP antibody (Cat#61884). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WTAP antibody (Cat#61884, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using WTAP antibody (Cat#61884). WTAP expression in wild-type (WT) and WTAP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with WTAP antibody (Cat#61884, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WTAP expression in HeLa cells using WTAP antibody (Cat#61884, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, WTAP.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WTAP
  • Catalog：
    61884
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated WTAP Mouse mAb
Aliases WTAP; WT1 Associated Protein; KIAA0105; Mum2; Wilms Tumor 1-Associating Protein; Wilms Tumor 1 Associated Protein; Pre-MRNA-Splicing Regulator WTAP; Female-Lethal(2)D Homolog; MGC3925; HFL(2)D; Putative Pre-MRNA Splicing Regulator Female-Lethal(2D); Wilms' Tumour 1-Associating Protein; WT1-Associated Protein; PNAS-132
Background

Gene Name: WTAP

NCBI Gene Entry: 9589

UniProt Entry: Q15007

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4070

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human WTAP
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using WTAP antibody (Cat#61884). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WTAP antibody (Cat#61884, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using WTAP antibody (Cat#61884). WTAP expression in wild-type (WT) and WTAP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with WTAP antibody (Cat#61884, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of WTAP expression in HeLa cells using WTAP antibody (Cat#61884, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, WTAP.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WTAP
  • Catalog：
    61884
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using WTAP antibody (Cat#61884). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WTAP antibody (Cat#61884, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using WTAP antibody (Cat#61884). WTAP expression in wild-type (WT) and WTAP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with WTAP antibody (Cat#61884, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WTAP expression in HeLa cells using WTAP antibody (Cat#61884, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, WTAP.
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