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KD-Validated XRCC6 Rabbit pAb

#61895

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shRNA Knockdown Validated
Western blotting analysis using XRCC6 antibody (Cat#61895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using XRCC6 antibody (Cat#61895). XRCC6 expression in wild-type (WT) and XRCC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    XRCC6
  • Catalog:
    61895
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated XRCC6 Rabbit pAb
Aliases XRCC6; X-Ray Repair Cross Complementing 6; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6; G22P1; KU70; ML8; X-Ray Repair Cross-Complementing Protein 6; Thyroid Autoantigen 70kDa (Ku Antigen); ATP-Dependent DNA Helicase 2 Subunit 1; Thyroid Autoantigen 70kD (Ku Antigen) ; 5'-Deoxyribose-5-Phosphate Lyase Ku70; Lupus Ku Autoantigen Protein P70; 70 KDa Subunit Of Ku Antigen; DNA Repair Protein XRCC6; Ku Autoantigen, 70kDa; 5'-DRP Lyase Ku70; D22S731; D22S671; CTC75; CTCBF; TLAA; ATP-Dependent DNA Helicase II, 70 KDa Subunit; ATP-Dependent DNA Helicase II 70 KDa Subunit; CTC Box Binding Factor 75 KDa Subunit; CTC Box-Binding Factor 75 KDa Subunit; Thyroid-Lupus Autoantigen P70; Ku Autoantigen P70 Subunit; Thyroid-Lupus Autoantigen; EC 4.2.99.-; EC 3.6.4.-; Ku70
Background

Gene Name: XRCC6

NCBI Gene Entry: 2547

UniProt Entry: P12956

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 75 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Ku70
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using XRCC6 antibody (Cat#61895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using XRCC6 antibody (Cat#61895). XRCC6 expression in wild-type (WT) and XRCC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using XRCC6 antibody (Cat#61895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using XRCC6 antibody (Cat#61895). XRCC6 expression in wild-type (WT) and XRCC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XRCC6 antibody (Cat#61895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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