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Home>Product>Primary Antibody

KD-Validated ACAD9 Mouse mAb

#61909

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Knockdown Validated
Western blotting analysis using ACAD9 antibody (Cat#61909). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ACAD9 antibody (Cat#61909, 1:1,000). Nuclei were stained blue with DAPI; ACAD9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of ACAD9 expression in HepG2 cells using ACAD9 antibody (Cat#61909, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ACAD9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACAD9
  • Catalog：
    61909
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ACAD9 Mouse mAb
Aliases ACAD9; Acyl-CoA Dehydrogenase Family Member 9; NPD002; Acyl-Coenzyme A Dehydrogenase Family, Member 9; Complex I Assembly Factor ACAD9, Mitochondrial; MGC14452; Very-Long-Chain Acyl-CoA Dehydrogenase VLCAD; Acyl-CoA Dehydrogenase Family, Member 9; EC 1.3.99.10; EC 1.3.8.-; EC 1.3.99; MC1DN20; ACAD-9
Background

Gene Name: ACAD9

NCBI Gene Entry: 28976

UniProt Entry: Q9H845

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 58 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4150

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ACAD9
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ACAD9 antibody (Cat#61909). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ACAD9 antibody (Cat#61909, 1:1,000). Nuclei were stained blue with DAPI; ACAD9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of ACAD9 expression in HepG2 cells using ACAD9 antibody (Cat#61909, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ACAD9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACAD9
  • Catalog：
    61909
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ACAD9 antibody (Cat#61909). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ACAD9 antibody (Cat#61909). ACAD9 expression in wild-type (WT) and ACAD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACAD9 antibody (Cat#61909, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ACAD9 antibody (Cat#61909, 1:1,000). Nuclei were stained blue with DAPI; ACAD9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of ACAD9 expression in HepG2 cells using ACAD9 antibody (Cat#61909, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ACAD9.
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