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Home>Product>Primary Antibody

KD-Validated RPS6KB1 Recombinant Rabbit mAb

#61931

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using RPS6KB1 antibody (Cat#61931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RPS6KB1 antibody (Cat#61931). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse brain using S6K1 antibody (Cat#61931, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    61931
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated RPS6KB1 Recombinant Rabbit mAb
Aliases Ribosomal Protein S6 Kinase B1; S6K1; P70(S6K)-Alpha; STK14A; PS6K; S6K; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase Beta-1; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Serine/Threonine Kinase 14 Alpha; P70 Ribosomal S6 Kinase Alpha; P70 S6 Kinase Alpha; P70 S6K-Alpha; P70-Alpha; P70-S6K 1; EC 2.7.11; P70-S6K; P70S6K1
Background

Gene Name: RPS6KB1

NCBI Gene Entry: 6198

UniProt Entry: P23443

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB5930

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-Paraffin (IHC-P)
Immunogen A synthesized peptide derived from human RPS6KB1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using RPS6KB1 antibody (Cat#61931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using RPS6KB1 antibody (Cat#61931). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using S6K1 antibody (Cat#61931, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    61931
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RPS6KB1 antibody (Cat#61931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RPS6KB1 antibody (Cat#61931). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KB1 antibody (Cat#61931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse brain using S6K1 antibody (Cat#61931, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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