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KD-Validated Ephrin A1 Recombinant Rabbit mAb

#61950

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Ephrin A1 antibody (Cat#61950). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Ephrin A1 antibody (Cat#61950). Ephrin A1 expression in wild type (WT) and Ephrin A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    EFNA1
  • Catalog:
    61950
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated Ephrin A1 Recombinant Rabbit mAb
Aliases EFNA1; Ephrin A1; LERK1; Ephrin-A1; TNFAIP4; ECKLG; EPLG1; GMAN ; Gastric Cancer Metastasis Associated Long Noncoding RNA; EPH-Related Receptor Tyrosine Kinase Ligand 1; Tumor Necrosis Factor Alpha-Induced Protein 4; Immediate Early Response Protein B61; TNF Alpha-Induced Protein 4; LERK-1; Tumor Necrosis Factor, Alpha-Induced Protein 4; Eph-Related Receptor Tyrosine Kinase Ligand 1; Epididymis Secretory Sperm Binding Protein; Ligand Of Eph-Related Kinase 1; EFL1; B61
Background

Gene Name: EFNA1

NCBI Gene Entry: 1942

UniProt Entry: P20827

Application Information

Molecular Weight: Predicted, 24 kDa; observed, 24 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5265

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Ephrin A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Ephrin A1 antibody (Cat#61950). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Ephrin A1 antibody (Cat#61950). Ephrin A1 expression in wild type (WT) and Ephrin A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Ephrin A1 antibody (Cat#61950). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Ephrin A1 antibody (Cat#61950). Ephrin A1 expression in wild type (WT) and Ephrin A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Ephrin A1 antibody (Cat#61950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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