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KD-Validated Baculoviral IAP Repeat Containing 2 Recombinant Rabbit mAb

#61996

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using BIRC2 antibody (Cat.#61996). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BIRC2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using baculoviral IAP repeat containing 2 antibody (Cat#61996). Baculoviral IAP repeat containing 2 expression in wild-type (WT) and baculoviral IAP repeat containing 2 (BIRC2) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with baculoviral IAP repeat containing 2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    BIRC2
  • Catalog:
    61996
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated Baculoviral IAP Repeat Containing 2 Recombinant Rabbit mAb
Aliases BIRC2; Baculoviral IAP Repeat Containing 2; C-IAP1; RNF48; MIHB; Hiap-2; CIAP1; API1; Baculoviral IAP Repeat-Containing Protein 2; RING-Type E3 Ubiquitin Transferase BIRC2; TNFR2-TRAF-Signaling Complex Protein 2; NFR2-TRAF Signalling Complex Protein; Cellular Inhibitor Of Apoptosis 1; Inhibitor Of Apoptosis Protein 2; RING Finger Protein 48; Apoptosis Inhibitor 1; IAP Homolog B; HIAP2; Baculoviral IAP Repeat-Containing 2; EC 2.3.2.27; HIAP-2; IAP-2
Background

Gene Name: BIRC2

NCBI Gene Entry: 329

UniProt Entry: Q13490

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 62 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7785

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human BIRC2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using BIRC2 antibody (Cat.#61996). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BIRC2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using baculoviral IAP repeat containing 2 antibody (Cat#61996). Baculoviral IAP repeat containing 2 expression in wild-type (WT) and baculoviral IAP repeat containing 2 (BIRC2) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with baculoviral IAP repeat containing 2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BIRC2 antibody (Cat.#61996). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BIRC2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using baculoviral IAP repeat containing 2 antibody (Cat#61996). Baculoviral IAP repeat containing 2 expression in wild-type (WT) and baculoviral IAP repeat containing 2 (BIRC2) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with baculoviral IAP repeat containing 2 antibody (Cat#61996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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