Western blotting analysis using Golgi integral membrane protein 4 antibody (Cat#62015). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Golgi integral membrane protein 4 antibody (Cat#62015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Golgi integral membrane protein 4 antibody (Cat#62015). Golgi integral membrane protein 4 expression in wild type (WT) and Golgi integral membrane protein 4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Golgi integral membrane protein 4 antibody (Cat#62015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with golgi integral membrane protein 4 antibody (Cat#62015, 1:1,000). Nuclei were stained blue with DAPI; Golgi integral membrane protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Golgi integral membrane protein 4 expression in HAP-1 cells using Golgi integral membrane protein 4 antibody (Cat#62015, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Golgi integral membrane protein 4.
Validation of Golgi integral membrane protein 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Golgi integral membrane protein 4 antibody (Cat#62015, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
