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Home>Product>Primary Antibody

KD-Validated Aurora Kinase A Recombinant Rabbit mAb

#62031

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using aurora kinase A antibody (Cat#62031). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using aurora kinase A antibody (Cat#62031). Aurora kinase A expression in wild-type (WT) and aurora kinase A (AURKA) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AURKA
  • Catalog：
    62031
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Aurora Kinase A Recombinant Rabbit mAb
Aliases AURKA; Aurora Kinase A; BTAK; ARK1; AIK; PPP1R47; STK15; AurA; STK7; STK6; Protein Phosphatase 1, Regulatory Subunit 47; Serine/Threonine-Protein Kinase Aurora-A; Serine/Threonine-Protein Kinase 6; Breast Tumor-Amplified Kinase; Aurora/IPL1-Related Kinase 1; Aurora 2; Serine/Threonine-Protein Kinase Ayk1; Serine/Threonine Protein Kinase 15; Serine/Threonine-Protein Kinase 15; Ipl1- And Aurora-Related Kinase 1; Serine/Threonine Kinase 15; Serine/Threonine Kinase 6; Aurora/IPL1-Like Kinase; Aurora-Related Kinase 1; Aurora-A Kinase; EC 2.7.11.1; AIRK1; ARK-1; AURA; AYK1; IAK1
Background

Gene Name: AURKA

NCBI Gene Entry: 6790

UniProt Entry: O14965

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7890

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Aurora kinase A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using aurora kinase A antibody (Cat#62031). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using aurora kinase A antibody (Cat#62031). Aurora kinase A expression in wild-type (WT) and aurora kinase A (AURKA) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AURKA
  • Catalog：
    62031
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using aurora kinase A antibody (Cat#62031). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using aurora kinase A antibody (Cat#62031). Aurora kinase A expression in wild-type (WT) and aurora kinase A (AURKA) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with aurora kinase A antibody (Cat#62031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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